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multiplex pcr master mix kit  (Qiagen)


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    Structured Review

    Qiagen multiplex pcr master mix kit
    Multiplex Pcr Master Mix Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5785 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+master+mix/QIAGEN+Multiplex+PCR+Kit/pm41903536-380-25-30
    Average 99 stars, based on 5785 article reviews
    multiplex pcr master mix kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Observation Bias in Metabarcoding.
    Article Snippet: .. The Qiagen Multiplex Master Mix had the largest negative effect (mean = −2.64E02 ± 1.45E- 03, 95% confidence interval: −0.0274, −0.02394), followed by Invitrogen Platinum Superfi (mean = −1.79E02 ± 1.46E- 03, 95% credible interval: −0.02035, −0.01548), Promega GoTaq Fusion (mean = −1.53E- 02 ± 1.37E- 03, 95% Actinopterygii 17550998, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/1755-0998.14119 by N at Prov Indonesia, W iley O nline L ibrary on [18/05/2025]. ..

    Article Title: Coastal Elites: Recurrent Trans‐Arctic Migration and Vicariance in a Cosmopolitan Sea Slug Driven by Pleistocene Glacial Cycles
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 4 of 19 Zoologica Scripta, 2025 amplifications was 10 μL, using 5.0 μL Qiagen Multiplex Master Mix, 0.4 μL BSA (10 mg/mL), 2.6 μL H2O, 1.0 μL primer mix and 1.0 μL template DNA. ..

    Article Title: Tracking genetic diversity in amur tigers: a long-term study using microsatellites in Southwest Primorye, Russia.
    Article Snippet: .. The PCR reactions were performed in a 10 μL mixture composed of 5 μL multiplex master mix (QIAGEN Multiplex PCR Kit), 0.5 μL Q solution (QIAGEN, Hilden, Germany), 1.0 μL (10 pmol) primer mix, and 2 μL template DNA. ..

    Article Title: Observation Bias in Metabarcoding.
    Article Snippet: .. When the mock community was amplified with the Qiagen Multiplex Master Mix, mismatches had the greatest negative effect on amplification efficiency (and the associated proportion of reads) compared to the other Taq polymerases. .. NEB Phusion HiFi had the least pronounced (but still) negative effect of mismatches.

    Article Title: Identifying a monozygotic twin brother as a donor of DNA in minimal, mixed forensic stains – a case example.
    Article Snippet: In forensic casework, monozygotic twins have always provided a challenge, as routinely used forensic Short Tandem Repeat (STR) profiles are not able to differentiate between the twin individuals.. In this study, we applied a method to discriminate between two monozygotic twin brothers in a sexual assault case that is unique and challenging for several reasons: the use of contact stains as evidence, the stains contain DNA from two persons (victim and one of the brothers), have minimal amounts of DNA, and there are PCR inhibiting factors.. Despite these challenging factors, we present a successfully solved case in which whole genome sequencing was applied to identify multiple somatic differences between the two brothers.

    Article Title: Spatio‐Temporal Variation in Diet Among Age and Sex Cohorts of a Model Generalist Bird Species, the Great Tit Parus major : New Insights Revealed by DNA Metabarcoding
    Article Snippet: .. In each 5 μL PCR, 2 μL of the extracted DNA was included initially, alongside 2.5 μL Multiplex Master Mix (Qiagen), 0.1 μL of each of the 10 μM forward and reverse primers (final concentration = 0.2 μM) and the remaining volume made up using nuclease‐free water. ..

    other:

    Article Title: Observation Bias in Metabarcoding.
    Article Snippet: FIGURE 6 | Differences in treatment on the proportion of reads for Actinopterygii species amplified with MiFishU, where treatments are control (no BSA, normal cycling conditions); +BSA (addition of BSA, normal cycling conditions); and TD- PCR (no BSA, TD PCR) for each Taq polymerase. (A) Mean proportion of reads (from triplicate samples) for each treatment, for each Taq polymerase used (IPSF = Invitrogen Platinum SuperFi; NPHF = NEB Phusion HiFi; PGTF = Promega GoTaq Flexi; QMMM = Qiagen Multiplex Master Mix). (B) Fitted parameters from zoid by treatment, with the intercept term given for the control treatment with NPHF in relation to Trachurus symmetricus in the first panel; and the change in that term given for each treatment in the next panels (whiskers show 95% posterior credibility interval for each effect; values of effect sizes on y- axis are in additive log- ratio space relative to the reference condition), with colours denoting the total number of mismatches to the forward and reverse primers (summed).

    Polymerase Chain Reaction:

    Article Title: Tracking genetic diversity in amur tigers: a long-term study using microsatellites in Southwest Primorye, Russia.
    Article Snippet: .. The PCR reactions were performed in a 10 μL mixture composed of 5 μL multiplex master mix (QIAGEN Multiplex PCR Kit), 0.5 μL Q solution (QIAGEN, Hilden, Germany), 1.0 μL (10 pmol) primer mix, and 2 μL template DNA. ..

    Article Title: Identifying a monozygotic twin brother as a donor of DNA in minimal, mixed forensic stains – a case example.
    Article Snippet: In forensic casework, monozygotic twins have always provided a challenge, as routinely used forensic Short Tandem Repeat (STR) profiles are not able to differentiate between the twin individuals.. In this study, we applied a method to discriminate between two monozygotic twin brothers in a sexual assault case that is unique and challenging for several reasons: the use of contact stains as evidence, the stains contain DNA from two persons (victim and one of the brothers), have minimal amounts of DNA, and there are PCR inhibiting factors.. Despite these challenging factors, we present a successfully solved case in which whole genome sequencing was applied to identify multiple somatic differences between the two brothers.

    Article Title: Spatio‐Temporal Variation in Diet Among Age and Sex Cohorts of a Model Generalist Bird Species, the Great Tit Parus major : New Insights Revealed by DNA Metabarcoding
    Article Snippet: .. In each 5 μL PCR, 2 μL of the extracted DNA was included initially, alongside 2.5 μL Multiplex Master Mix (Qiagen), 0.1 μL of each of the 10 μM forward and reverse primers (final concentration = 0.2 μM) and the remaining volume made up using nuclease‐free water. ..

    Amplification:

    Article Title: Observation Bias in Metabarcoding.
    Article Snippet: .. When the mock community was amplified with the Qiagen Multiplex Master Mix, mismatches had the greatest negative effect on amplification efficiency (and the associated proportion of reads) compared to the other Taq polymerases. .. NEB Phusion HiFi had the least pronounced (but still) negative effect of mismatches.

    Concentration Assay:

    Article Title: Identifying a monozygotic twin brother as a donor of DNA in minimal, mixed forensic stains – a case example.
    Article Snippet: In forensic casework, monozygotic twins have always provided a challenge, as routinely used forensic Short Tandem Repeat (STR) profiles are not able to differentiate between the twin individuals.. In this study, we applied a method to discriminate between two monozygotic twin brothers in a sexual assault case that is unique and challenging for several reasons: the use of contact stains as evidence, the stains contain DNA from two persons (victim and one of the brothers), have minimal amounts of DNA, and there are PCR inhibiting factors.. Despite these challenging factors, we present a successfully solved case in which whole genome sequencing was applied to identify multiple somatic differences between the two brothers.

    Article Title: Spatio‐Temporal Variation in Diet Among Age and Sex Cohorts of a Model Generalist Bird Species, the Great Tit Parus major : New Insights Revealed by DNA Metabarcoding
    Article Snippet: .. In each 5 μL PCR, 2 μL of the extracted DNA was included initially, alongside 2.5 μL Multiplex Master Mix (Qiagen), 0.1 μL of each of the 10 μM forward and reverse primers (final concentration = 0.2 μM) and the remaining volume made up using nuclease‐free water. ..



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